How to use pipettes

Pipettes are calibrated « to deliver ». The first stage is to fill the pipette for filling process you always have to use a pipette filler or a pipette pump. Fill the liquid max 10mm above the volume mark. Then you must wipe and make dry the outer surface of the pipette. Hold the pipette to your eye level and place the tip of the pipette to the wall of a vessel with a slight angle. Then you have to discharge the access volume until meniscus reaches the calibration mark of the pipette. Second step is to empty yhe pipette. You must empty the pipette by placing the tip of the pipette to the wall of the vessel with a slight angle. Pay attention to empty the pipette completely (or until the 2nd mark of bottom graduation line).